Transportin 1

PDB rendering based on 1qbk.

Available structures
PDB	1QBK, 2H4M, 2OT8, 2QMR, 2Z5J, 2Z5K, 2Z5M, 2Z5N, 2Z5O

Identifiers

Symbols

TNPO1; IPO2; KPNB2; MIP; MIP1; TRN

External IDs

OMIM: 602901 MGI: 2681523 HomoloGene: 5358 GeneCards: TNPO1 Gene

Gene Ontology
Molecular function	• protein binding • nuclear localization sequence binding • protein transporter activity
Cellular component	• nucleus • cytoplasm • cytosol
Biological process	• protein import into nucleus, translocation • RNA metabolic process • mRNA metabolic process • interspecies interaction between organisms
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 5:
72.11 – 72.21 Mb

Chr 13:
99.61 – 99.7 Mb

PubMed search

[1]

[2]

Transportin-1 is a protein that in humans is encoded by the TNPO1 gene.^[1]^[2]^[3]

This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins.^[3]

Interactions

Transportin 1 has been shown to interact with RGPD5^[4] and Ran (biology).^[5]^[6]

References

^ Pollard VW, Michael WM, Nakielny S, Siomi MC, Wang F, Dreyfuss G (Dec 1996). "A novel receptor-mediated nuclear protein import pathway". Cell 86 (6): 985–94. doi:10.1016/S0092-8674(00)80173-7. PMID 8808633.
^ Bonifaci N, Moroianu J, Radu A, Blobel G (Jun 1997). "Karyopherin β2 mediates nuclear import of a mRNA binding protein". Proc Natl Acad Sci U S A 94 (10): 5055–60. doi:10.1073/pnas.94.10.5055. PMC 24630. PMID 9144189. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=24630.
^ ^a ^b "Entrez Gene: TNPO1 transportin 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3842.
^ Cai, Y; Miao S Y, Wang L F (Oct. 2001). "[Determination of the binding site of testis-specific nucleoporin BS-63 to transportin (karopherin beta 2) and the proof of their combination in vitro]". Zhongguo Yi Xue Ke Xue Yuan Xue Bao (China) 23 (5): 462–6. ISSN 1000-503X. PMID 12905863.
^ Chook, Y M; Blobel G (May. 1999). "Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp". Nature (ENGLAND) 399 (6733): 230–7. doi:10.1038/20375. ISSN 0028-0836. PMID 10353245.
^ Shamsher, Monee K; Ploski Jonathan, Radu Aurelian (Oct. 2002). "Karyopherin β2B participates in mRNA export from the nucleus". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (22): 14195–9. doi:10.1073/pnas.212518199. ISSN 0027-8424. PMC 137860. PMID 12384575. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=137860.

Bukrinsky MI, Haffar OK (2004). "HIV-1 nuclear import: in search of a leader". Front. Biosci. 2: d578–87. PMID 9366553.
Bukrinsky MI, Haffar OK (1998). "HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr". Mol. Med. 4 (3): 138–43. PMC 2230352. PMID 9562972. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2230352.
Bukrinsky MI, Sharova N, Dempsey MP, et al. (1992). "Active nuclear import of human immunodeficiency virus type 1 preintegration complexes". Proc. Natl. Acad. Sci. U.S.A. 89 (14): 6580–4. doi:10.1073/pnas.89.14.6580. PMC 49545. PMID 1631159. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=49545.
Moroianu J, Hijikata M, Blobel G, Radu A (1995). "Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins". Proc. Natl. Acad. Sci. U.S.A. 92 (14): 6532–6. doi:10.1073/pnas.92.14.6532. PMC 41552. PMID 7604027. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=41552.
Bukrinsky MI, Haggerty S, Dempsey MP, et al. (1993). "A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells". Nature 365 (6447): 666–9. doi:10.1038/365666a0. PMID 8105392.
Michael WM, Choi M, Dreyfuss G (1996). "A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway". Cell 83 (3): 415–22. doi:10.1016/0092-8674(95)90119-1. PMID 8521471.
Nakielny S, Siomi MC, Siomi H, et al. (1997). "Transportin: nuclear transport receptor of a novel nuclear protein import pathway". Exp. Cell Res. 229 (2): 261–6. doi:10.1006/excr.1996.0369. PMID 8986607.
Fridell RA, Truant R, Thorne L, et al. (1997). "Nuclear import of hnRNP A1 is mediated by a novel cellular cofactor related to karyopherin-beta". J. Cell. Sci. 110 ( Pt 11): 1325–31. PMID 9202393.
Gallay P, Hope T, Chin D, Trono D (1997). "HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway". Proc. Natl. Acad. Sci. U.S.A. 94 (18): 9825–30. doi:10.1073/pnas.94.18.9825. PMC 23276. PMID 9275210. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=23276.
Siomi MC, Eder PS, Kataoka N, et al. (1997). "Transportin-mediated Nuclear Import of Heterogeneous Nuclear RNP Proteins". J. Cell Biol. 138 (6): 1181–92. doi:10.1083/jcb.138.6.1181. PMC 2132560. PMID 9298975. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2132560.
Henderson BR, Percipalle P (1998). "Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta". J. Mol. Biol. 274 (5): 693–707. doi:10.1006/jmbi.1997.1420. PMID 9405152.
Efthymiadis A, Briggs LJ, Jans DA (1998). "The HIV-1 Tat nuclear localization sequence confers novel nuclear import properties". J. Biol. Chem. 273 (3): 1623–8. doi:10.1074/jbc.273.3.1623. PMID 9430704.
Vodicka MA, Koepp DM, Silver PA, Emerman M (1998). "HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection". Genes Dev. 12 (2): 175–85. doi:10.1101/gad.12.2.175. PMC 316441. PMID 9436978. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=316441.
Popov S, Rexach M, Zybarth G, et al. (1998). "Viral protein R regulates nuclear import of the HIV-1 pre-integration complex". EMBO J. 17 (4): 909–17. doi:10.1093/emboj/17.4.909. PMC 1170440. PMID 9463369. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170440.
Popov S, Rexach M, Ratner L, et al. (1998). "Viral protein R regulates docking of the HIV-1 preintegration complex to the nuclear pore complex". J. Biol. Chem. 273 (21): 13347–52. doi:10.1074/jbc.273.21.13347. PMID 9582382.
Fouchier RA, Meyer BE, Simon JH, et al. (1998). "Interaction of the Human Immunodeficiency Virus Type 1 Vpr Protein with the Nuclear Pore Complex". J. Virol. 72 (7): 6004–13. PMC 110405. PMID 9621063. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=110405.

PDB gallery

1qbk: STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX

2h4m: Karyopherin Beta2/Transportin-M9NLS

2ot8: Karyopherin Beta2/Transportin-hnRNPM NLS Complex

Transportin 1

Interactions

References

Further reading